Our Platform · OrganoKit
OrganoKit keeps organoids in place through every media change
High-precision organoid culture chip and kit
OrganoKit is AiderBio's first product. It is a slide-format 3D culture kit with 18 independent wells, each built around our Port Pocket structure and a honeycomb microwell array.
It fits a standard microscope slide holder, so cultures can be imaged at every time point without being transferred.
Port Pocket system
A separate pocket takes the pipette tip, so the force of the media stream never reaches the organoids.
Honeycomb microwells
Closely packed microwells promote aggregation and give organoids of uniform size.
3D zone separation
The culture and pipetting zones sit at different heights, removing flow interference during media exchange.
Coating-free
Start 3D culture right away, without an extracellular matrix pre-coating step.
How the Port Pocket works
The pipette tip enters a pocket beside the culture zone. Fresh media rises over a partition and spreads slowly across the culture zone, while organoids stay settled in their microwells below.
Patent application No. 10-2026-0112592.
Culture images
Cell aggregates in Port Pocket microwells (brightfield) and A549-GFP cells in 3D culture (fluorescence), from AiderBio's culture tests.
A549-GFP, 1×10⁴ / 5×10³ cells per 20 µL, day 10–12, 4× and 10×
Specifications
| Model | OrganoKit |
|---|---|
| Product | 3D organoid culture kit |
| Wells | 18 independent wells |
| Material | High-purity bio-grade silicone, transparent |
| Dimensions | 76 × 25 × 15 mm (standard slide footprint) |
| Weight | 5 g |
| Sterilization | Gamma irradiated, individually packed |
| Coating | Coating-free, no ECM pre-coating needed |
| Contents | OrganoKit body, sterile cover, manual |
| Made in | AiderBio Co., Ltd., Republic of Korea |
Compared with other 3D culture formats
Figures for existing formats are from published literature. OrganoKit figures are development targets at the prototype stage.
| Format | Strength | Limitation | CV | Cell recovery | Detachment |
|---|---|---|---|---|---|
| Standard 3D plates | Easy and low cost | Structures disturbed by media change | 20–30% | 50–60% | 40–50% |
| Hydrogel kits | Tissue-like matrix | Losses during gel digestion and recovery | 20–30% | 50–65% | 20–30% |
| Organ-on-chip | Physiological flow | Costly, complex to run | 15–25% | 60–70% | 15–25% |
| OrganoKit | Culture, recovery and analysis in one format | Validation in progress | 10–15% | 70–85% | 5–10% |
Zhu et al., Sci. Rep. 15, 9142 (2025) · Piergiovanni et al., Lab Chip 21, 2857 (2021) · Caliari & Burdick, Nat. Methods 13, 405 (2016) · Kozlowski et al., Commun. Biol. 4, 1387 (2021) · Velázquez-García et al., Sci. Rep. 9, 6199 (2019) · Wardwell-Swanson et al., InSphero white paper (2021) · Li et al., Protein Cell 16 (2025)